اثر درمانی سیکلوسپورین بر آسیب دیررس ریوی ناشی از گاز خردل در موش

Background and Aim: Among late complications in mustard gas or sulfur mustard (SM) casualties, respiratory problems, especially chronic bronchitis and pulmonary fibrosis are the greatest causes of longterm disability. Cyclosporine has anti-inflammatory effects and is used in other pulmonary fibroses due to other factors. Thus, with respect to the role of oxidative stress in the pathogenesis of chronic bronchitis and pulmonary fibrosis, the therapeutic effects of Cyclosporine on delayed complications of mustard gas and the role of oxidative stress in the procedure were studied. Materials and Methods: In this experimental study, a group of six male mice received cyclosporine after five months of inhaling mustard gas. After killing the mice and obtaining tissue samples, biochemical analyses of hydroxyproline of lung, H2O2, vitamin C of serum, and also alveolar space percentage and inflammatory cell infiltration, the comparison between positive and negative control and also between case and positive control was performed. Results: Results indicated a significant decrease in alveolar space percent, lymphocyte infiltration, H2O2, and vitamin C in the positive control group. Cyclosporine administration decreased lung hydroxyproline and lymphocyte infiltration; and normalized H2O2 and vitamin C. Conclusion: Decrease of pulmonary alveolar space of the mice brings up two hypotheses. First: collagen precipitation and incidence of pulmonary fibrosis. Second: inflammatory cell infiltration and incidence of chronic inflammation that is corresponding to SM induced lung injury in chemical casualties. Decrease of H2O2 -that can be due to decrease of superoxide dismutase- and vitamin C, demonstrates decrease of antioxidant defense in the serum of the mice. Cyclosporine administration effects by normalizing these two agents-; i.e decreasing oxidative stress- induces a decrease in lung hydroxyproline (index of fibrosis) and lymphocyte infiltration (index of chronic tissue inflammation).